Composite

Part:BBa_K3258012:Design

Designed by: Alec Lourenco   Group: iGEM19_Stanford   (2019-10-16)


lacI-derived biosensor selection schema (negative selection) PREDCEL+


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 240
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 115
    Illegal BamHI site found at 1028
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 240
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 240
    Illegal NgoMIV site found at 13
    Illegal AgeI site found at 997
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source varies - it is a mixture of viral (T3 coliphage) and E. coli derived


Source

Sequence synthesized via Twist.

References